RESEARCH CAREER

General interests: Evolutionary genomics; genomics of thermal stress and ecological clines; molecular populations genetics

Dissertation topic: Ecological and evolutionary genomics of the northern acorn barnacle (Semibalanus balanoides)

Senior thesis topic: Population genetics using complete mitochondrial genomes from population of Fundulus heteroclitus distributed along a pollution cline

8/2015 – Present:

Ecological and evolutionary genomics of the northern acorn barnacle across ocean basins

Brown University

P.I. Dr. David Rand

The northern acorn barnacle (S. balanoides) has wide regional gene flow and strong patterns of selection in local habitats. Moreover, this organism is exposed to annual cycles of reproduction and settlement that may limit local adaptation in favor of adaptation to environmental heterogeneity. This project utilizes barnacles as models for ecological genomics in order to gain greater understanding of the genome-by-environment interactions involved in adaptations to highly heterogeneous environments.

This project in the media

Barnacles offer genetic clues on how organisms adapt to changing environments

By Phoebe Hall, Assistant Director of BioMedical Communications, Division of Biology and Medicine

Support for this Project:

“Ontogenetically mediated selection in response to environmental heterogeneity in the acorn barnacle (Semibalanus balanoides)”, Doctoral Dissertation Enhancement Grant (DDEG), Brown University, Dept. of Ecology and Evolutionary Biology. US $10,000; 2/1/2019 - 2/1/2020. Lead PI(s) JCB Nunez and DM Rand

 

“Evolutionary Genomics of the Northern Acorn Barnacle (Semibalanus balanoides)”, Graduate Research Fellowship (GRFP). National Science Foundation (NSF), US $138,000; 05/1/2015 - 05/1/2020. Lead PI(s) JCB Nunez

 

“Parallel evolution in the intertidal: investigating genetic responses to zonation", Graduate Research Opportunities Worldwide (GROW). A joint grant from the U.S. National Science Foundation (NSF), and the Swedish Research Council (Vetenskapsrådet), US $5,000 and SE kr 26,000. 7/2019 – 10/2019. Lead PI(s) JCB Nunez, DM Rand, K Johannesson and A Blomberg. 

“Tidally-zonated polymorphisms in the northern acorn barnacle in the North Atlantic: parallel evolution or ancient polymorphism?” KVA fund for internationalization and scientific renewal at the Sven Lovén Centre.The Royal Swedish Academy of Sciences(KVA), SE kr 64,100; 12/21/2018 - 12/1/2019. Lead PI(s) JCB Nunez and K Johannesson 

"NSF IGERT: Reverse Ecology: Computational Integration of Genomes, Organisms, and Environments." NSF training grant from DGE 0966060 to P.I Dr. David Rand (JCB Nunez supported under IGERT fellowship 2015-2017)

Barnacle zonation. Winter 2017

Characterizing thermal stress variation in the intertidal (Nunez et al 2020 PNAS)

Predicting protein structure differences of a key metabolic gene (Mpi) in the intertidal (Nunez et al 2020 PNAS)

Papers from this Project:

[1.] Joaquin C. B. Nunez, Patrick A. Flight, Kimberly B. Neil, Stephen Rong, Leif A. Eriksson, David A. Ferranti, Magnus Alm Rosenblad, Anders Blomberg, and David M. Rand (2020) Footprints of natural selection at the mannose-6-phosphate isomerase locus in barnacles

PNAS first published February 25, 2020 https://doi.org/10.1073/pnas.1918232117

[2.] Nunez J.C.B., Elyanow R.G., Ferranti D.A., Rand D.M. (2018) Population Genomics and Biogeography of the Northern Acorn Barnacle (Semibalanus balanoides) Using Pooled Sequencing Approaches. In Population Genomics: Marine Organisms. M. F. O. and O. P. Rajora, Springer, Cham. DOI:https://doi.org/10.1007/13836_2018_58

NSF Highlights

6/2013 – 6/2015:

Mitochondrial genomics of Fundulus heteroclitus 

Rosenstiel School Of Marine And Atmospheric Science
P.I.s Dr. Marjorie Oleksiak and Dr. Douglas Crawford

We utilized next generation sequencing approaches to obtain several complete mitochondrial genomes from populations of Fundulus heteroclitus distributed along ecological clines, particularly a pollution cline and a thermal cline. Our goal is to utilize SNPs found in mitochondrial the genome to find correlations between mitochondrial haplotypes and ecological adaptation.

Support for this Project:

“Evolutionary Genomics of the Mitochondrial Genome in Fundulus”, Small Undergraduate Research Grant Experience (SURGE). Rosenstiel School of Marine and Atmospheric Science, Amount: US $1500; 1/20/2015 - 5/1/2015. Lead PI(s) JCB Nunez 

 

“Searching for signatures of natural selection in the mitochondrial genome in Fundulus heteroclitus”, Small Undergraduate Research Grant Experience (SURGE). Rosenstiel School of Marine and Atmospheric Science, Amount: US $1500; 1/20/2014 - 5/1/2014. Lead PI(s) JCB Nunez 

Papers from this Project:

[1.] Nunez JCB, Biancani LM, Flight PA, Nacci DE, Rand DM, Crawford DL, Oleksiak MF. 2018 Stable genetic structure and connectivity in pollution-adapted and nearby pollution-sensitive populations of Fundulus heteroclitus. Royal Society Open Science 5: 171532. http://dx.doi.org/10.1098/rsos.171532

[2.] Nunez JCB, Oleksiak MF (2016) A Cost-Effective Approach to Sequence Hundreds of Complete Mitochondrial Genomes. PLoS ONE 11(8): e0160958. doi: 10.1371/journal.pone.0160958

As one of our findings, we observed an enrichment of non-synonymous mutations on the mtDNAs of fish living in the most polluted area of the New Bedford estuary, MA (Nunez et al 2018 RSOS)

M. Oleksiak and me  (2015)

1/2014 – 7/2014:

Population genetics of the euryhaline teleost Poecilia latipinna

Rosenstiel School Of Marine And Atmospheric Science.

P.I.s Dr. Marjorie Oleksiak and Dr. Douglas Crawford

We used a genotyping by sequencing (GBS) approach to examined hundreds of SNPs to determine the genetic diversity of Sailfin molly (Poecilia latipinna) among three geographically close mangrove salt marsh flats in the Florida Keys and compare this to populations in southern and northern Florida.

Support for this Project:

Department of Marine Biology and Ecology, University of Miami - RSMAS, Undergraduate program in Marine Biology

Papers from this project:

[1.] Nunez, J. C. B., Seale, T. P., Fraser, M. A., Burton, T. L., Fortson, T. N., Hoover, D., Travis, J., Oleksiak, M. F., Crawford, D. L. (2015). “Population Genomics of the Euryhaline Teleost Poecilia latipinna.” PLoS ONE 10(9): e0137077.

Population structure in south florida mollies (Nunez et al 2015)

 D. Crawford (background) and me collecting sailfin mollies. Pic by T. Seale 2014

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© 2020 by Joaquin C. B. Nunez

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